Journal article
Redefining the human corneal immune compartment using dynamic intravital imaging
LE Downie, X Zhang, M Wu, S Karunaratne, JK Loi, K Senthil, S Arshad, K Bertram, AL Cunningham, N Carnt, SN Mueller, HR Chinnery
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 2023
Abstract
The healthy human cornea is a uniquely transparent sensory tissue where immune responses are tightly controlled to preserve vision. The cornea contains immune cells that are widely presumed to be intraepithelial dendritic cells (DCs). Corneal immune cells have diverse cellular morphologies and morphological alterations are used as a marker of inflammation and injury. Based on our imaging of corneal T cells in mice, we hypothesized that many human corneal immune cells commonly defined as DCs are intraepithelial lymphocytes (IELs). To investigate this, we developed functional in vivo confocal microscopy (Fun-IVCM) to investigate cell dynamics in the human corneal epithelium and stroma. We show..
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Awarded by Royal Victorian Eye and Ear Hospital
Funding Acknowledgements
From the University of Melbourne, we thank Ching Yi Wu for the CD3 immunostaining (Fig. 5), Haihan Jiao for assisting with the CX3CR1-eGFP time-lapsed studies, and Rajni Rajan who contributed to image analyses. We acknowledge the Florey Microscopy Facility, The Florey Institute of Neuroscience and Mental Health, for instrumentation, training, and general support. Flow cytometry was performed at the Westmead Scientific Platforms, supported by the Westmead Research Hub, Westmead Institute for Medical Research, Cancer Institute New South Wales, National Health and Medical Research Council, and the Ian Potter Foundation. We acknowledge the Lions Eye Donation Service-Centre for Eye Research Australia Biobank, Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, University of Melbourne.